Fig. 5
From: Development of an In Vitro 3D Model for Investigating Ligamentum Flavum Hypertrophy
Protein expression in LF cells in 2D culture and 3D culture. One thousand spheroid cells were cultured for 7 days, and collagen I, collagen III, elastin and fibronectin were detected on days 4 and 7. a The expression of collagen I and III was determined by Western blotting. b, c Quantitative evaluation of (a). d The expression of fibronectin and elastin was determined by Western blotting. e, f Quantitative evaluation of (d). The data represent the mean ± SD. “*” denotes statistical significance with a p value < 0.05 among the experimental groups, whereas “**” denotes statistical significance with a p value < 0.01. Equal protein loading was verified by GAPDH