Fig. 6

Assessment of the purity level on the apoptotic bodies isolation method. The dot graphs show the flow-cytometry data analysis of isolated apoptotic bodies preparations from a total of 12 patients, 4 diagnosed with ischemic stroke, 4 diagnosed with multiple sclerosis and 4 patients with Parkinson’s disease, and from 6 healthy volunteers. The amounts of the total membrane vesicles, stained positively with Annexin V (AnnV) (filled black symbols), of the apoptotic bodies population, measured as double positive counts for annexin V and PI (empty pink symbols), and of the non-apoptotic vesicles population showing a annexin V-positive and a PI-negative signal (empty black symbols), expressed as the absolute number of each vesicle subsets counted in 50 μl-volume of the apoptotic bodies fractions, are represented (a). Representative flow cytometry profiles of signal intensity in dual staining for Annexin V and propidium iodide of the apoptotic bodies preparations from neurological patients and from controls. The relative percentage of gated AnnexinV⁺PI⁺ apoptotic bodies found in the isolated vesicles samples is indicated (b)