Table 4 Primer sequences used and amplicon sizes of Nup107 and tin for RFLP
From: Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach
Nup107 | tin | |
|---|---|---|
Forward primer | GAGCAGAATGTCTCGGTGCT | ATTCCGATGCTGTGCTGTGATTG |
Reverse primer | AGGAAGAGCACTATGTGGGC | TTAAATAAGTCCAACAATTTGCC |
PCR product length (bp) | 512 | 498 |
Digestion enzyme (recognition site) | ApaI (GGGCC^C) | NlaIII (CATG^) |
Digested product size (bp, WT/mutant) | 311 + 201/512 | 412 + 86 / 348 + 86 + 64 |