Table 2 Sequences for ssODN design. PAM is underlined, gRNA DSB site is indicated by a slash (/), and the introduced mutations locations are indicated in BOLD
From: Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach
Nup107 | tin | |
|---|---|---|
Locus | Chr2L (-) | Chr3R (+) |
Genomic sequence | GCCGATTCCAAGAACTATGACGAGTACAGCCGCGCGACGGCGGGTGTCTTCTCCGGCCACTTGGGCTCGCTGAAAACCCTTTTGCACAGCAACTGGCACGATTTGCTCTGGGCCCATC/TGAAGGTGCAGATCGACATCCGTGTGGAATCGGAGATACGCGGCTGCTGCCTCAAAAACTACCAACCGATGCCCGATGATT | TGAGGCGGTCTGGGGTGAAAGGAGTACGCGTTCAGTACCAAAATCGAGCTGACAAATTGCAG/ACACGGTCGAGTGCGCATCGGAACGGCGCTTTACGGCTTACGGGTTACGGACTACTGATTGCCGATTACGGGC |
gRNA target | TTTGCTCTGGGCCCATCTGAAGG | GAGCTGACAAATTGCAGACACGG |
Designed ssODN | GCCGATTCCAAGAACTATGACGAGTACAGCCGCGCGACGGCGGGTGTCTTCTCCGGCCACTTGGGCTCGCTGAAAACCCTTTTGCACAGCAACTGGCACaATTTGCTCTGGGCtCAcCTGAAGGTGCAGATCGACATCCGTGTGGAATCGGAGATACGCGGCTGCTGCCTCAAAAACTACCAACCGATGCCCGATGATT | TGAGGCGGTCTGGGGTGAAAGGAGTACGCGTTCAGTACCAAAATCGAGCTGACAAATTGCctcatgtGTCGAGTGCGCATCGGAACGGCGCTTTACGGCTTACGGGTTACGGACTACTGATTGCCG |