Fig. 2
From: Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach
Detailed crossing schemes for chromosome 2 and 3. Detailed crossing schemes followed to generate aNup107D364N and btinmDPE mutant strains. The relevant balancer strains are indicated for each chromosome, as well as the selection criteria in each generation. The injected flies harbor the mutation in the germ cells, and therefore no phenotype is expected in the hatched P flies. Note that the genotype shown for the P flies represents their germ cells and not their soma. Final strains are expected to be either homozygote or heterozygote for the mutation, based on its lethality. In crosses where the P founder fly was a male, the family eye color was determined based only on the females’ eye phenotype, as all F1 progeny males carry the w− allele found on the maternal X chromosome