Fig. 5
From: Bone Marrow-Derived Mesenchymal Stem Cells Modified with Akt1 Ameliorates Acute Liver GVHD
AKT1-MSCs ameliorated ConA-induced liver injury. Female C57BL/6 mice were intravenously injected with PBS (negative control), Null-MSCs (5×106), or AKT1-MSCs (5×106) 30min after ConA (20mg/kg) administration. a Serum levels of ALT and AST were measured at 6h, 12h, and 24h after MSCs injection. b Representative photographs of H&E-stained liver tissue in each group were shown. Scale bar = 100μm. c The expression of TNF-α and INF-γ in serum was measured by ELISA at 6h, 12h and 24h after MSCs injection. d Intrahepatic TNF- α and INF-γ protein levels were measured by immunohistochemical staining at 6h, 12h and 24h after MSCs injection. e The expression quantifications of TNF- α and INF-γ were analyzed by Image-Pro Plus 6.0. IOD (Integrated Option Density) was checked out by calculating the area and the density of the brown color in IHC staining. The data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001