DNA Methylation Validation Methods: a Coherent Review with Practical Comparison

Table 5 Costs summary of each method

Method	Total cost of analysis [$]	Number of samples measured	Number of standards measured	Total number of measurements	Cost per measurement [$]
MSRE analysis	576	10 for each locus^*, Test and Reference reaction, duplicates	2 for each locus^*, duplicates	144	4
pyrosequencing	162^‡	10 for each locus^*	2 for each locus^*	36	4.5
MS-HRM	85	10 for each locus^*, duplicates	6 for each locus^*, duplicates	96	0.9
qMSP	196	10 for each primer set^†, duplicates	2 for each primer set^†, duplicates	216	0.9

ISSN: 1480-9222