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Table 2 Correlation coefficients for peak height-based MS-HRM calibration curves and counted methylation levels

From: DNA Methylation Validation Methods: a Coherent Review with Practical Comparison

Locus name

Primer set

R2

Methylation [%]

(n = 10)

± SD

M

M HRM

0.952

93.61

5.28

M

M HRM Wojdacz

0.798

85.49

5.13

IM

IM HRM

0.973

29.20

4.71

U

U HRM

0.868

2.69

1.04

U

U HRM Wojdacz

0.938

0.57

0.81

  1. M methylated locus, IM intermediately methylated locus, U unmethylated locus, R2 square of the correlation coefficient, SD standard deviation.
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Biological Procedures Online

ISSN: 1480-9222

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