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Table 4 Intra-assay precision(repeatability)test

From: Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products

Standard(pg/ml)

Mean CT(n = 3)

Standard Deviation

C.V.(%)

300

14.50

0.021

0.145

30

17.78

0.015

0.084

3

21.41

0.014

0.065

0.3

25.05

0.062

0.248

0.03

28.5

0.061

0.214

0.003

32.09

0.145

0.452