From: Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products
Standard(pg/ml)
Mean CT(n = 3)
Standard Deviation
C.V.(%)
300
14.50
0.021
0.145
30
17.78
0.015
0.084
3
21.41
0.014
0.065
0.3
25.05
0.062
0.248
0.03
28.5
0.061
0.214
0.003
32.09
0.452