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Fig. 2 | Biological Procedures Online

Fig. 2

From: A simple and reliable protocol for long-term culture of murine bone marrow stromal (mesenchymal) stem cells that retained their in vitro and in vivo stemness in long-term culture

Fig. 2

Long term cultured BMSCs-FS differentiates efficiently into adipocytic cell lineage. a Adipocyte differentiation of BMSCs-FS (p25) as compared with ST2 cell line. Cells were induced with adipogenic cocktail (M&M) and accumulation of fat droplets during the time course of differentiation were measured by quantitative Oil Red O staining. Representative images of Oil Red O staining are shown at each time point during adipogenesis of BMSCs-FS and ST2 cells. b qPCR analysis of the adipogenic markers expression in BMSCs-FS and ST2 cells after 12 days of adipogenic induction. Data were represented as fold change over control non-induced. Values are mean ± SD of three independent experiments, (*p < 0.05, compared to ST2 cells at the same time points). Columns of the same group with different letters at each time point indicate significant differences according to Duncan’s multiple range tests at p < 0.05

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