Table 2 Qualitative assessment of fluorescent staining of tight junction antibodies, where: - indicates negative staining; + indicates weak staining with no consistent structure; ++ indicates moderate staining of tight junctions, with some structure present; +++ indicates strong staining, with consistent structures present
From: Visualisation of Multiple Tight Junctional Complexes in Human Airway Epithelial Cells
Fixation | Claudin-1 | Occludin | ZO-1 |
|---|---|---|---|
4% Paraformaldehyde | – | – | – |
0.2% Triton Pre-extraction + 4% Paraformaldehyde | – | – | – |
4% Paraformaldehyde + 0.1% Saponin | – | – | + |
4% Paraformaldehyde + Acetone | – | – | – |
Methanol | +++ | – | +++ |
0.2% Triton Pre-extraction + Methanol | – | – | – |
Methanol + 0.1% Saponin | + | – | + |
Methanol + Acetone | – | +++ | +++ |
Acetone | – | – | – |