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Table 2 Qualitative assessment of fluorescent staining of tight junction antibodies, where: - indicates negative staining; + indicates weak staining with no consistent structure; ++ indicates moderate staining of tight junctions, with some structure present; +++ indicates strong staining, with consistent structures present

From: Visualisation of Multiple Tight Junctional Complexes in Human Airway Epithelial Cells

Fixation Claudin-1 Occludin ZO-1
4% Paraformaldehyde
0.2% Triton Pre-extraction + 4% Paraformaldehyde
4% Paraformaldehyde + 0.1% Saponin +
4% Paraformaldehyde + Acetone
Methanol +++ +++
0.2% Triton Pre-extraction + Methanol
Methanol + 0.1% Saponin + +
Methanol + Acetone +++ +++
Acetone