Fig. 3From: Visualisation of Multiple Tight Junctional Complexes in Human Airway Epithelial CellsMethanol-acetone (1:1) fixation of 16HBE14o- cells in culture. The top row of panels show the absence of claudin-1 staining, whilst ZO-1 staining is clearly visible. The bottom row of panels show co-staining of occludin with ZO-1 using methanol-acetone as the fixative. Merged images showed nuclei staining with Hoechst (blue)Back to article page