Fig. 2From: Visualisation of Multiple Tight Junctional Complexes in Human Airway Epithelial CellsMethanol fixation of 16HBE14o- cells in culture. The top row of panels show claudin-1 co-stained with ZO-1. The bottom row of panels show the destruction of occludin staining using methanol as the fixative. Merged images showed nuclei staining with Hoechst (blue)Back to article page