Fig. 5

T cell degranulation upon challenge with tumor cells. MoDCs from HLA-A*02 donors were loaded with MCF-7 whole tumor cell lysates (TL). Then, these MoDCs were used to stimulate autologous T cells. Co-culture took place for 3 weeks (1 MoDC: 5 T cells), with a MoDCs re-stimulus after 1 week of co-culture. Each set of MoDCs (MACS or EasySep) was cultured with the respective CD14⁻ fraction. After 3 weeks, T cells were co-cultured with the MCF-7 breast tumor cell line for 5 h, and T cell degranulation was assessed by flow cytometry. Control conditions with tumor cells and unstimulated T cells (Tu + T) and tumor cells with T cells stimulated with unloaded MoDCs (Tu + T + MoDC) are also presented in the graph. Data refers to the mean percentage of CD107a⁺ cells ± SEM from 4 different donors. Asterisks represent statistical significance (**p < 0.01)