Skip to main content
Fig. 4 | Biological Procedures Online

Fig. 4

From: Production of Monoclonal Antibodies against Human Trefoil Factor 3 and Development of a Modified-Sandwich ELISA for Detection of Trefoil Factor 3 Homodimer in Saliva

Fig. 4

Sandwich ELISA for the measurement of TFF3 homodimer. The sandwich ELISA was developed using a pair of mAbs against TFF3. The three combinations of the capture and detector mAbs, namely TFF-116 and FITC-labeled TFF-116 (116–116 FITC), TFF-286 and FITC-labeled TFF-286 (286–286 FITC) and TFF-298 and FITC-labeled TFF-298 (298–298 FITC), were employed for detecting recombinant TFF3 monomeric and dimeric forms. Bar graphs represent mean ± SD of two independent experiments. There was statistically significantly higher reactivity in dimeric form compared with monomeric and no antigen (all P-values <0.05)

Back to article page

Biological Procedures Online

ISSN: 1480-9222

Contact us