Skip to main content

Table 1 Sequencing results of cross-linked and native ChIP-seq on muscle samples. Two biological replicates were sequenced for each chromatin immunoprecipitation method (X-ChIP and N-ChIP). Reads were mapped against the chicken genome Galgal5, and peaks were detected using epic. When indicated (40 M), analyses were performed on 40 million of unique reads per sample that were randomly selected for both H3K27me3 and input fractions. Genome coverage is expressed in Megabases

From: An Assessment of Fixed and Native Chromatin Preparation Methods to Study Histone Post-Translational Modifications at a Whole Genome Scale in Skeletal Muscle Tissue

Method X-ChIP N-ChIP
Sample X_R1 X_R2 N_R1 N_R2
Fraction Input H3K27me3 Input H3K27me3 Input H3K27me3 Input H3K27me3
Total number of reads (millions) 57.37 119.58 60.29 123.37 57.98 92.93 58.94 128.56
Total number of uniquely mapped reads (millions) 51.16 106.36 52.55 104.29 45.17 69.58 46.51 92
Total peak number 2127 2519 16,446 13,949
Total genome coverage (Mb) 7.87 9.75 73.52 71.14
40 M peak number 1264 1484 14,128 11,900
40 M genome coverage (Mb) 4.76 5.21 60.51 51.21