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Table 1 Sequencing results of cross-linked and native ChIP-seq on muscle samples. Two biological replicates were sequenced for each chromatin immunoprecipitation method (X-ChIP and N-ChIP). Reads were mapped against the chicken genome Galgal5, and peaks were detected using epic. When indicated (40 M), analyses were performed on 40 million of unique reads per sample that were randomly selected for both H3K27me3 and input fractions. Genome coverage is expressed in Megabases

From: An Assessment of Fixed and Native Chromatin Preparation Methods to Study Histone Post-Translational Modifications at a Whole Genome Scale in Skeletal Muscle Tissue

Method

X-ChIP

N-ChIP

Sample

X_R1

X_R2

N_R1

N_R2

Fraction

Input

H3K27me3

Input

H3K27me3

Input

H3K27me3

Input

H3K27me3

Total number of reads (millions)

57.37

119.58

60.29

123.37

57.98

92.93

58.94

128.56

Total number of uniquely mapped reads (millions)

51.16

106.36

52.55

104.29

45.17

69.58

46.51

92

Total peak number

2127

2519

16,446

13,949

Total genome coverage (Mb)

7.87

9.75

73.52

71.14

40 M peak number

1264

1484

14,128

11,900

40 M genome coverage (Mb)

4.76

5.21

60.51

51.21