Fig. 3
From: Pre-Microporation Improves Outcome of Pancreatic Islet Labelling for Optical and 19F MR Imaging
Images of pancreatic islets labelled with fluorine nanoparticles using microporation according to the standard protocol (a), endocytosis (b), pre-microporation (c), and unlabelled controls (d). 19F (red) MR images superimposed on 1H (grey scale) MR images (upper row), fluorescence images (middle) and selected microphotographs of islets stained for the viability test. Selected voltages (600, 800, 1000 V) used for microporation or pre-poration are shown. Unporated control represents unlabelled islets incubated without any intervention in the pure medium. Porated control contains islets porated in the buffer only (600 V) and incubated without nanoparticles