From: Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins
Buffer | Components | pH value |
---|---|---|
PBS | 140Â mM NaCl, 2.7Â mM KCl, 10Â mM Na2HPO4 and 1.8Â mM KH2PO4 | 7.4 |
High pH buffer | 0.1Â M Tris-HCl and 0.5Â M NaCl | 8.5 |
Low pH buffer | 0.1Â M sodium acetate, 0.5Â M NaCl | 4.5 |
Exchange buffer | 50Â mM Tris-HCl and 10Â mM reduced glutathione | 8.5 |