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Table 1 Buffers used in the study

From: Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins

Buffer

Components

pH value

PBS

140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4 and 1.8 mM KH2PO4

7.4

High pH buffer

0.1 M Tris-HCl and 0.5 M NaCl

8.5

Low pH buffer

0.1 M sodium acetate, 0.5 M NaCl

4.5

Exchange buffer

50 mM Tris-HCl and 10 mM reduced glutathione

8.5