Fig. 1

System design and screening of IDLV. Schematic representation of the HIV-1 integrase domain and its basic region (a). Numbers correspond to amino acid position of integrease domain. Site-directed mutagenesis resulted in mutation of the wild-type amino acids (bold black letters) to mutants (bold red letters). An illustration of a lenti-reporter used to assess the transduction and expression of each mutant. The reporter has dual promoter, CMV and EF1α, MCS are multiple cloning sites for potential insertion of gene of interest. PolyA isthe signaling sequences of polyadenylation (b). HT1080 cells were imaged after 72 h transduction and the best performer in mutant group was highlighted in red (c)