Table 2 Oligonucleotides used in this study
From: Lambda Red-mediated Recombineering in the Attaching and Effacing Pathogen Escherichia albertii
Primers | Purpose | Sequence |
|---|---|---|
SB2454 | 5′ primer for replacing ler with cat | GATTAGGTCATTAATAGCTTAATATATTAAAGCATGCGGAGATTATTTATcatatgaatatcctcctta |
SB2455 | 3′ primer for replacing ler with cat | TATCGTTATCATCTAATGGTTTTATATTAAATATTTTTCAGCGGCATTAAgtgtaggctggagctgcttc |
SB2456 | 5′ primer to verify ler deletion | gcg GAATTCCCTTTAGCTCCTGGCACTTTTGAAC |
SB2457 | 3′ primer to verify ler deletion | gcg AAGCTTTGACGTAATATTTTATTCAGCTGAT |
SB2464 | 5′ primer for replacing grlRA with kan | TGGATAGAACAAATTGAAAGGAGTGAGGTTGGTATGAAACTGAGTGAGTTgtgtaggctggagctgcttc |
SB2467 | 3′ primer for replacing grlRA with kan | ATGTATGTGAAAAGTTATGTCTAACTCCCTTTTTTCCGTCTCATGATCATttccggggatccgtcgacct |
SB2470 | 5′ primer to verify grlRA deletion | gcg GAATTCTGAGCCTGTGGCACAATTGAT |
SB2471 | 3′ primer to verify grlRA deletion | gcg AAGCTTGCAGTCTGATGAAGTGATCCC |
SB2481 | 5′ primer for replacing hfq with cat | TCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAcatatgaatatcctcctta |
SB2482 | 3′ primer for replacing hfq with cat | AAAAACAGCCCGAAACCTTATTCGGTTTCTTCGCTGTCCTGTTGCGCGGAgtgtaggctggagctgcttc |
SB2485 | 5′ primer to verify hfq deletion | gcg GAATTCGCTATCGCAGGCTGAATGTGT |
SB2486 | 3′ primer to verify hfq deletion | gcg AAGCTTACCAGCATCATAACGGTCAAA |