Table 1 Bacterial strains and plasmids used in this study

LS5494

Escherichia albertii Albert 19982^T

Manan Sharma

LS5504

LS5494(pSIM6), Amp^R (Ts)

This study

LS5568

LS5494∆ler::cat, Cm^R Clone #1

This study

LS5570

LS5494∆ler::cat, Cm^R Clone #2

This study

LS5592

LS5568(pFT-K), Cm^R Kan^R (Ts)

This study

LS5593

LS5570(pFT-K), Cm^R Kan^R (Ts)

This study

LS5598

LS5494∆grlRA::kan, Kan^R

This study

LS5607

LS5494∆ler::FRT = LS5592∆cat ∆pFT-K, Cm^S Kan^S

This study

LS5609

LS5494∆ler::FRT = LS5593∆cat ∆pFT-K, Cm^S Kan^S

This study

LS5612

LS5494∆hfq::cat, Cm^R

This study

DH5α

supE44 ∆lacU169 (Φ80 lacZ∆M15) hsdR17 recA1 endA1 gryA96 thi-1 relA1

Bettina Bommarius

Plasmids

pSIM6

p _L -gam-bet-exo genes under the control of CI857 repressor, Amp^R (Ts)

Don Court

pKD46

p _araBAD -gam-bet-exo under the control of AraC, Amp^R (Ts)

Barry Wanner

pKD3

Template plasmid to amplify FRT-cat-FRT amplicon, Amp^R Cm^R

Barry Wanner

pKD13

Template plasmid to amplify FRT-kan-FRT amplicon, Amp^R Kan^R

Barry Wanner

pFT-K

flp gene under the control of Tet^R repressor, Kan^R (Ts)

Fred Blattner