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Table 1 Overview of elution volumes and molar masses of Mistic/alkyl maltoside complexes under native and denaturing conditions

From: Characterising protein/detergent complexes by triple-detection size-exclusion chromatography

0 M urea

Sample

Elution volume (mL)

M w,comp (kg/mol)

M w,prot (kg/mol)

M w,det (kg/mol)

  Mistic + DDM

9.8

53

13

40

  Mistic + UM

10.0

47

13

34

  Mistic + DM

10.3

33

13

20

 

10.8

38

0

38

  Mistic + NM

10.5

25

13

12

 

11.5

28

0

28

6 M urea

Sample

Elution volume (mL)

M w,comp (kg/mol)

M w,prot (kg/mol)

M w,det (kg/mol)

  Mistic + DDM

9.3

0

13*

30*

  Mistic + UM

9.3

12

12

0

 

10.0

23

0

23

  Mistic + DM

9.3

13

13

0

 

10.8

19

0

19

  Mistic + NM

9.3

13

13

0

 

13.5

9

0

9

  1. *Values derived from linear combination of independent Mistic and DDM measurements rather than global analysis