Fig. 3
From: Characterising protein/detergent complexes by triple-detection size-exclusion chromatography
SEC as monitored by static light scattering of different alkyl maltosides in the (a) absence and (b) presence of 6 M urea. Excess Rayleigh ratios at 90° and molar masses of DDM, UM, DM, and NM are depicted as functions of elution volume. 50 mM Tris, pH 7.4, 50 mM NaCl, c det,sample = CMC8 M urea + 5 mM, c det,buffer = CMCbuffer + 2 mM, flow rate 0.4 mL/min, RT