Fig. 3

Restriction digest data qualified by fragment length analyses. a-d Collated percentages of homozygous edited, heterozygous and homozygous un-edited clones in each cell line. For each cell line, the top graph shows the restriction digest data and the bottom graph shows the fragment length analysis data. The actual numbers of clones analyzed are shown on top of each bar. The pie chart at the bottom of each cell line panel indicates the zygosity of the clones analyzed by the combined approaches of restriction digest and PCR fragment length analyses. Note that RD cells have 3 copies of EZH2, whereas G-401, G-402 and KYM-1 cells have two copies