Fig. 7
in vitro validation of single PNA-based FMCA for detection and discrimination between HRV and other Rhabdovirus species. The amplification plot showed quantitative detection of serially diluted targets. The melt points were converted to distinct melting points by plotting the first negative derivative of fluorescence as a function of temperature (−dF/dT). Products of different sequences were melted at different temperatures and observed as distinct points at 69 °C, 58 °C, and 39 °C)