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Fig. 6 | Biological Procedures Online

Fig. 6

From: Detection of genetic variation using dual-labeled peptide nucleic acid (PNA) probe-based melting point analysis

Fig. 6

Modifiability of PNA probe Tm. a Melting points of PNA probes depend on probe length and (b) the number of gamma-modified PNA(s). Melting points of perfectly matched probes (upper panel) and single mismatch (center panel) and merged melting points (lower panel) are listed. Green lines represent perfect match and red lines represent single mismatch of each probe. Probe and target sequences are listed at the bottom of the figure. Underline, mismatch point; Red sequences, gamma-modified PNA

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