Fig. 5

Quantitative analysis of PNA–FMCA system. Amplification points of real-time PCR reaction of FMCA conditions were measured at the primer annealing step of PCR reactions. a Amplification points and (b) standard point of each dilutant with four replicants were measured with (c) melting points and (d) melting points with four replicants in a closed well reaction. Orange lines represent negative controls