Fig. 1

PNA probe-based FMCA for mutation detection. Schematic model of PNA probe-based FMCA for detection of SNP, deletion, and insertion. Mismatch or partial hybridization makes probe melting temperature lower than that of perfectly matched probe. At the denatured temperature, the PNA probe undergoes fluorescence quenching by random coiling and this quenching temperature was analyzed by the real-time PCR machine. Blue line, perfectly matched sequence; Green line, mismatched or unmatched sequence; Red line, inserted sequence; Q, quencher; F, Fluorescence