Fig. 6
Protein level expression of VEGF165, MMP2 and MMP9 on CAM under SaOS2 cells. Immunohistochemical images of CAM incubated with 20 μl volume of 12 × 105 cells and control with DMEM alone for 96 h. CAM incubated with SaOS2 cells (d and f) shows increased intensity for MMP2 and MM9 in the chorionic layer and also at the stroma region and VEGF 165 at the vessel endothelium of the capillaries located beneath the chorionic layer (b) than control. Control CAM (c and e) shows less staining for MMP2 and MMP9 throughout the chorionic layer and substantial staining for VEGF A (a) at the capillaries. Arrow indicates the presents of protein, magnification is 40× and bar is 50 μM