Fig. 5
SaOS2 cells interact with CAM vasculature. Scanning electron microscope images of CAM incubated with 20 μl volume of 12 × 105 cells and control with DMEM alone for 96 h of incubation. CAM incubated with SaOS2 cells shows more number of angiogenic holes, multiple cell junctions along with numerous sprouting vessels (b). Control CAM shows normal morphological structure with minimum angiogenic holes (a). Images are representative of 3 set of experiments and bar is = 200 μm. (a) for angiogenic holes, (b) for multiple cell junctions and (c) for sprouting vessels