Figure 5

Epic® response is strain-dependent. The response was measured after addition of increasing concentrations of rHA from different influenza A and B strains. The ability of each rHA to agglutinate turkey red blood cells is reflected in the HAU provided after each strain name: B/Wisconsin/1/2010 (1024 HAU/μg), B/BR/08 (2048 HAU/μg), A/Brisbane/59/2007 (H1N1) (2048 HAU/μg), A/Vietnam/1203/2004 (H5N1) (1024 HAU/μg), A/Anhui/1/2013 (H7N9) (4 HAU/μg) and A/Hangzhou/2013 (H7N9) (32HAU/μg). The assays used bovine fetuin immobilized under optimal conditions at 150 μg/ml. The results of binding to asialofetuin were all negative (no response after addition of rHA).