Table 1 Total score for the six commercially available kits, based on three parameters: a) success rate score, b) ABL1 score and c) linearity
From: cDNA synthesis for BCR-ABL1 detection at the MMR level: the importance of using the appropriate kit
Product name | Success rate score | ABL1 score | Linearity score | Total score |
|---|---|---|---|---|
AffinityScript Multiple Temperature cDNA Synthesis Kit | 2 | 3 | 6 | 11 |
(Product number: 200436, Agilent Technologies) | ||||
iScriptâ„¢ Select cDNA Synthesis Kit | 1 | 1 | 2 | 4 |
(Product Number: 170–8897, Bio-Rad) | ||||
SuperScript® III Reverse Transcriptase | 6 | 6 | 6 | 18 |
(Product Number: 18080–044, Invitrogen) | ||||
QuantiTect® Reverse Transcription Kit | 5 | 2 | 4 | 11 |
(Product Number: 205311, Qiagen) | ||||
First Strand cDNA Synthesis Kit for RT-PCR (AMV) | 5 | 5 | 4 | 14 |
(Product Number: 11 483 188 001, Roche Applied Sciences) | ||||
Transcriptor First Strand cDNA Synthesis Kit | 5 | 4 | 2 | 11 |
(Product Number: 04 379 012 001, Roche Applied Sciences) |