Figure 2

Comparison of fresh lymphoid tissue sections cut with a Compresstome or Vibratome. Fresh unfixed lymph node (A-B) and spleen (C-D) tissue sections were generated with either a Compresstome (left panels) or Vibratome (right panels) and processed for in situ tetramer staining combined with immunohistochemistry. Tissue sections were stained with Mamu-A1/Gag (CM9) MHC-tetramers to label SIV-specific CD8 T cells (red to pinkish color), anti-CD20 antibodies to detect B cells (green), and anti-CD3 antibodies to detect T cells (Blue). Confocal mages were collected using a 20 X objective and 3 um z-steps. The upper left panel of each image shows a montage of several projected confocal z-series fields. Scale bars = 100 microns. We used the curves tool in Photoshop to increase the contrast of each image, and each image was adjusted similarly.