Figure 5
From: Detection and quantification of extracellular microRNAs in murine biofluids
Absolute quantification of synthetic miR-1 oligonucleotides. A 10-fold dilution series of synthetic miR-1 oligonucleotides was diluted in 20 ng/μl yeast tRNA. RNA was extracted from each standard and reverse transcribed using stem-loop primers. The resulting cDNA was analysed by small RNA TaqMan RT-qPCR. The top standard contains 3 million copies and the bottom standard contains 300 copies. (a) Amplification plot. (b) Standard curve.