Table 3 Name of the DNA fragments, the nucleotide sequence used for designing the PCR primers and product length
From: Simultaneous splicing of multiple DNA fragments in one PCR reaction
DNA fragments name | Primer name | Primer sequences | Product length (bp) |
|---|---|---|---|
D1 | D1F | 5′gaattcACCCGGGTGGGCATCG3′ | 2000 |
D1R | 5′CCGGCTTTTCATATAgaattcGAATAGAGAAGCGTTCATGAC3′ | ||
D2 | D2F | 5′GTCATGAACGCTTCTCTATTCgaattcTATATGAAAAGCCGG3′ | 1500 |
D2R | 5′GAAGATCCTTTTTGATgaattcAATCTCATGACCAAAATC3′ | ||
D3 | D3F | 5′GATTTTGGTCATGAGATTgaattcATCAAAAAGGATCTTC3′ | 1000 |
D3R | 5′CCGCTCATGAGACAgaattcATAACCCTGATAAATG3′ | ||
D4 | D4F | 5′CATTTATCAGGGTTATgaattcTGTCTCATGAGCGG3′ | 750 |
D4R | 5′GTAGAGGTCGAGTTTgaattcAGATGCAAGTTCAAGG3′ | ||
D5 | D5F | 5′CCTTGAACTTGCATCTgaattcAAACTCGACCTCTAC3′ | 500 |
D5R | 5′GAATCTATACTTCTTgaattcTTTTGTTCTACAAAAATG3′ | ||
D6 | D6F | 5′CATTTTTGTAGAACAAAAgaattcAAGAAGTATAGATTC3′ | 200 |
D6R | 5′GACCTGCAGGCATGCAgaattcGCTGCATTTTTACAG3′ | ||
D7 | D7F | 5′CTGTAAAAATGCAGCgaattcTGCATGCCTGCAGGTC3′ | 100 |
D7R | 5′gaattcACCCTTATATTTGTC3′ |