Table 1 Concentration of DNA measured by UV spectrophotometry, PicoGreen, and qPCR
From: Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods
| Â | NanoDrop | |||||
|---|---|---|---|---|---|---|
| Â | Undiluted samples | 10-fold diluted samples | Â | Â | ||
|  | Mean ± SD | p value | Mean ± SD | p value |  |  |
intact DNA | 45.18 ± 9.99 |  | 7.77 ± 1.10 |  |  |  |
1500 bp | 41.39 ± 9.17 | *** | 7.62 ± 1.30 | ns |  |  |
500 bp | 41.59 ± 8.76 | *** | 7.26 ± 1.11 | ns |  |  |
150 bp | 44.41 ± 9.13 | ns | 7.70 ± 1.00 | ns |  |  |
| Â | PicoGreen | |||||
| Â | 10-fold diluted samples | 100-fold diluted samples | 1000-fold diluted samples | |||
|  | Mean ± SD | p value | Mean ± SD | p value | Mean ± SD | p value |
intact DNA | 39.19 ± 7.79 |  | 6.19 ± 0.72 |  | 1.79 ± 0.20 |  |
1500 bp | 37.07 ± 7.85 | ns | 5.70 ± 0.72 | *** | 1.62 ± 0.17 | *** |
500 bp | 34.24 ± 7.62 | *** | 5.27 ± 0.68 | *** | 1.46 ± 0.19 | *** |
150 bp | 27.73 ± 5.68 | *** | 4.41 ± 0.44 | *** | 1.20 ± 0.08 | *** |
| Â | qPCR | |||||
| Â | 10-fold diluted samples | 100-fold diluted samples | 1000-fold diluted samples | |||
|  | Mean ± SD | p value | Mean ± SD | p value | Mean ± SD | p value |
intact DNA | 5.42 ± 1.52 |  | 0.46 ± 0.17 |  | 0.04 ± 0.02 |  |
1500 bp | 6.16 ± 1.79 | ns | 0.50 ± 0.16 | ns | 0.04 ± 0.01 | ns |
500 bp | 4.92 ± 1.79 | ns | 0.42 ± 0.12 | ns | 0.03 ± 0.01 | ns |
150 bp | 1.78 ± 0.69 | *** | 0.15 ± 0.04 | *** | 0.02 ± 0.01 | *** |