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Table 7 Multimer PCR conditions

From: A do-it-yourself protocol for simple transcription activator-like effector assembly

Cycle Number Denature Anneal Extend
1 95°C, 2 min   
2-36 95°C, 20 s 60°C, 20 s 72°C, 30 s
37    72°C, 3 min
Hold at 4°C