Table 7 Multimer PCR conditions
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Cycle Number | Denature | Anneal | Extend |
|---|---|---|---|
1 | 95°C, 2 min |  |  |
2-36 | 95°C, 20 s | 60°C, 20 s | 72°C, 30 s |
37 |  |  | 72°C, 3 min |
Hold at 4°C |  |  |  |