Table 7 Multimer PCR conditions
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
| Cycle Number | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 95°C, 2 min | ||
| 2-36 | 95°C, 20 s | 60°C, 20 s | 72°C, 30 s |
| 37 | 72°C, 3 min | ||
| Hold at 4°C |
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
| Cycle Number | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 95°C, 2 min | ||
| 2-36 | 95°C, 20 s | 60°C, 20 s | 72°C, 30 s |
| 37 | 72°C, 3 min | ||
| Hold at 4°C |