Table 5 Thermocycle conditions for exonuclease treatment
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Cycle Number | Temperature 1 | Temperature 2 |
|---|---|---|
1 | 37°C, 30 min | 70°C, 30 min |
Hold at 4°C |  |  |
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Cycle Number | Temperature 1 | Temperature 2 |
|---|---|---|
1 | 37°C, 30 min | 70°C, 30 min |
Hold at 4°C |  |  |