Table 3 Thermocycle conditions for multimer assembly
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Cycle Number | Temperature 1 | Temperature 2 |
|---|---|---|
1 | 37°C, 5 min |  |
2-16 | 20°C, 5 min | 37°C, 5 min |
Hold at 4°C |  |  |
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Cycle Number | Temperature 1 | Temperature 2 |
|---|---|---|
1 | 37°C, 5 min |  |
2-16 | 20°C, 5 min | 37°C, 5 min |
Hold at 4°C |  |  |