Figure 6

Microdialysis setup and operation. a) Ringer’s solution is perfused into the MD probe, and the sample is passed to a flow cell being continuously analyzed by a UV-Vis spectrometer. Absorption data is recorded and the dialysate continues to the waste container. b) The probe is inserted into the subcutaneous tumor on the mouse and fixed with surgical tape. c) The tip of the probe has Ringer’s solution continually flowing at a rate of 2 μ l/min, allowing small molecules (e.g. MMC) to pass into the dialysate and be carried to the flow cell for analysis.