Figure 4

Real-time monitoring of intratumor MMC via microdialysis. All plots show the evolution of the magnitude of the MMC (blue line) and blood absorption (red line). There is a latency time of 4-5 min from the tissue fluid is sampled till it reaches the detector. In a) the probe is installed and the tumor is injected with MMC three times with and without electroporation (EP). In b) a second EP treatment is performed after the initial treatment, and a small MMC signal is observed, despite no additional MMC being injected. In c) an EP treatment without an MMC injection shows that EP alone does not produce an MMC signal. Plot d) shows an EP treatment without MMC injection, as well as the probes being placed on the tumor without application of voltage pulses. This shows that it is the physical manipulation of the tumor that is transferring MMC within the tumor, and that the measured MMC signal is unrelated to EP.