Figure 3

The robustness of dual reporters. (a and b, upper panels): Images of TALE-induced GFP reporter expression in HEK293 cells. (a) Low-range of Sox2-TALE-TF input, images taken at 20x magnification (b) High-dose range of Sox2-TALE-TF input, images taken at 10x magnification. (a and b, lower panels): Levels of luciferase activities expressed as mean ± SD, n = 3 at low-dose (a) and high-dose (b) range of Sox2-TALE-TF input. The fold induction of the reporter was calculated as relative activity of each dosage compared to the corresponding control (dual reporter plus empty TALE-TF vector).