Enhancement of anti-murine colon cancer immunity by fusion of a SARS fragment to a low-immunogenic carcinoembryonic antigen

Table 1 Comparison of the CEA and CEA-SARS epitopes with other known epitopes that have been proven to elicit immunity in BALB/c mice.

Gene	Primer	Sequence (5'→3')	Tm (°C)
	P1	TACggAATTCATggAgTCTCCCTCggCCCCTCCCCACAgATggTgCATCC	71.6
	P2	CCTggCAgAggCTCCTgCTCACAggTgAAgggAggACAAC	71.5
	P3	CTgggAgAgggTgggAGggAgggAgCTggggTCTCCTgggT	75.1
CEA	P4	CTCCTCCCACCCTCTCCCAggTTgTCCTCCCTTCACCTgT	71.8
	P5	gAgCAggAgCCTCTgCCAggggATgCACCATCTgTggggA	73.7
	P6	gCTATC TAgATCACAgCCCTgTCCTACCCAggAgACCCCAgCTCC	71.1
	P7	gCTATCTAgACAgCCCTgTCCTACCCAggAgACCCCAgCTCC	71.0
	P1	TACgTCTAgAAAAgTCgAggCggAggTACAAATTgACAggTTAATTACA	65.7
	P2	ggCAgACTTCAAAgCCTTCAAACCTATgTAACACAACAAC	63.9
	P3	TAATCAggATTAAATggCCTTggTATgTTTggCTCggCTT	65.3
SARS	P4	CATTgCTggACTAATTgCCATCgTCATggTTACAATCTTg	63.7
	P5	gCTAAAgCTTTTAAgTCATgCAACAAAgCAAgATTgTAACCATgACgA	64.1
	P6	TggCAATAgTCCAgCAATgAAgCCgAgCCAAACATACCA	67.7
	P7	AggCCATTTAATCCTgATTAgTTgTTgTgTTACATggTT	61.7
	P8	TgAAggCTTTgAAgTCTgCCTgTAATTAACCTGgTCAATTT	63.2

ISSN: 1480-9222