Table 1 Comparison of the CEA and CEA-SARS epitopes with other known epitopes that have been proven to elicit immunity in BALB/c mice.
Gene | Primer | Sequence (5'→3') | Tm (°C) |
|---|---|---|---|
P1 | TACggAATTCATggAgTCTCCCTCggCCCCTCCCCACAgATggTgCATCC | 71.6 | |
P2 | CCTggCAgAggCTCCTgCTCACAggTgAAgggAggACAAC | 71.5 | |
P3 | CTgggAgAgggTgggAGggAgggAgCTggggTCTCCTgggT | 75.1 | |
CEA | P4 | CTCCTCCCACCCTCTCCCAggTTgTCCTCCCTTCACCTgT | 71.8 |
P5 | gAgCAggAgCCTCTgCCAggggATgCACCATCTgTggggA | 73.7 | |
P6 | gCTATC TAgATCACAgCCCTgTCCTACCCAggAgACCCCAgCTCC | 71.1 | |
P7 | gCTATCTAgACAgCCCTgTCCTACCCAggAgACCCCAgCTCC | 71.0 | |
P1 | TACgTCTAgAAAAgTCgAggCggAggTACAAATTgACAggTTAATTACA | 65.7 | |
P2 | ggCAgACTTCAAAgCCTTCAAACCTATgTAACACAACAAC | 63.9 | |
P3 | TAATCAggATTAAATggCCTTggTATgTTTggCTCggCTT | 65.3 | |
SARS | P4 | CATTgCTggACTAATTgCCATCgTCATggTTACAATCTTg | 63.7 |
P5 | gCTAAAgCTTTTAAgTCATgCAACAAAgCAAgATTgTAACCATgACgA | 64.1 | |
P6 | TggCAATAgTCCAgCAATgAAgCCgAgCCAAACATACCA | 67.7 | |
P7 | AggCCATTTAATCCTgATTAgTTgTTgTgTTACATggTT | 61.7 | |
P8 | TgAAggCTTTgAAgTCTgCCTgTAATTAACCTGgTCAATTT | 63.2 |