Table 2 Primer Sequences for EGFR and GAPDH Transcripts used for Real-Time Quantitative reverse Transcriptase Polymerase Chain Reaction
From: Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells
Name | Forward | exon | Sequence | Locationa | Reverseb | exon | Sequence | LocationΔ | Length(bp) |
|---|---|---|---|---|---|---|---|---|---|
q1 | EGFR 370F | 2 | GGCACTTTTGAAGATCATTTTCTC | c.370_393 | EGFR 514R | 3 | CTGTGTTGAGGGCAATGAG | c.514_532 | 163 |
q2 | EGFR 1089F | 7 | CGAGGGCAAATACAGCTT | c.1089_1106 | EGFR 1263R | 9 | AAATTCACCAATACCTATT | c.1263_1281 | 193 |
q3 | EGFR 2034F | 15 | GGCAGGAGTCATGGGAGAA | c.2034_2052 | EGFR 2168R | 17 | GCGATGGACGGGATCTTAG | c.2168_2186 | 153 |
GAPDH 240F | 3 | TTGCCATCAATGACCCCTTCA | c.240_260 | GAPDH 395R | 5 | CGCCCCACTTGATTTTGGA | c.395_413 | 173 | |
GAPDH | GAPDH 820F | 8 | TGAACGGGAAGCTCACTGG | c.820_837 | GAPDH 1106R | 9 | TCCACCACCCTGTTGCTGTA | c.1106_1125 | 306 |
GAPDH 1016F | 8 | ACCCACTCCTCCACCTTTG | c.1016_1034 | GAPDH 1175R | 9 | CTCTTGTGCTCTTGCTGGG | c.1175_1193 | 177 |