Fig. 1
From: Influence of RT-qPCR Primer Position on EGFR Interference Efficacy in Lung Cancer Cells
Determination of EGFR siRNA knockdown efficiency in H358 cells with RT-qPCR. a Schematic diagram of the EGFR gene exon (boxes) structure and location of siRNAs and RT-qPCR primer sets. For the siRNAs sequences, see Table 1. For the primers sequences, see Table 2. Primer sets were named according to the order of the sequences. b EGFR mRNA knockdown efficiency detected by RT-qPCR amplification from EGFR siRNA-treated H358 cells using either the primer set q1,q2 and q3 at 48 h (left), and at 72 h (right). Transfections were performed in triplicate