Figure 3

HQ antagonizes the effect of TNF-α-induced NF-κB production in pIκB–EGFP-transfected 293 cells. Transfected 293 cells were added either with TNF-α (100 μM) or TNF-α plus HQ (100 μM). After 24-h incubation, the amount of IκB–EGFP proteins (shown as fluorescence intensity) was measured by flow cytometry (results expressed as mean ± standard deviation).