Figure 1

The accumulation of IκB–EGFP proteins in pIκB–EGFP-transfected Huh7 cells caused by microbial metabolite-derived antioxidant. a The representative histogram pattern of pIκB–EGFP-transfected cell with or without antioxidant treatment. b Antioxidants including HQ, PA, β-PPA, and HA (100 μM) were added into pIκB–EGFP-transfected Huh 7 cells. Growth medium was also added into another well as control. After 24-h incubation of the antioxidants, the amount of IκB–EGFP proteins (shown as fluorescence intensity) was measured by flow cytometry (results expressed as mean ± standard deviation).