Quick and Simple Detection Technique to Assess the Binding of Antimicrotubule Agents to the Colchicine-Binding Site

Table 2 Competition assay of different drugs with N,N'-ethylene-bis(iodoacetamide) on HT-29, M21, and MCF-7 and their effects on the inhibition of the bisthioalkylation of Cys-239 and Cys-354 of β-tubulin by N,N'-ethylene-bis(iodoacetamide) using SDS 65%

Compound	Drug treatment	Competition assay			Inhibition of EBI binding to β-tubulin
	(µM)	HT-29	M21	MCF-7
Dimethylsulfoxide	0.5%				N/A
EBI	100				N/A
Colchicine + EBI	2.5				Yes
Combretastatin A-4 + EBI	2.5				Yes
Podophyllotoxin + EBI	20				Yes
Daunorubicin + EBI	50				No

ISSN: 1480-9222