Table 2 LDH absorbance changes at 340 nm as a function of time in the presence or absence of Rottlerin
From: Critical Appraisal of the MTT Assay in the Presence of Rottlerin and Uncouplers
DMSO | 20 μM Rottlerin | |||
|---|---|---|---|---|
Time | OD | Δ A | OD | Δ A |
30 s | 1.248 | 1.365 | ||
1.5 min | 1.211 | 0.037 | 1.320 | 0.045 |
2.5 min | 1.180 | 0.031 | 1.296 | 0.024 |
3.5 min | 1.147 | 0.033 | 1.262 | 0.034 |
4.5 min | 1.122 | 0.025 | 1.222 | 0.040 |
Mean ΔA/min | 0.031 | 0.035 | ||
LDH (U/l) | 503 | 568 | ||