Figure 4
Constructed lacZ reporter strains, SMdC-PnlmAB and SMGS5-PnlmAB, express predicted β-gal activity in response to CSP or agonist SPA-18. a The expression of β-gal activity in SMdC-PnlmAB is CSP-dependent, which is in contrast to strain SMGS5-PnlmAB. b A time-course experiment indicates that SMdC-PnlmAB expresses the highest levels of β-gal activity around 90 min after the addition of CSP or SPA-18 and maintains such high levels of the activity for at least 2 h.